Evaluation of indirect sequence-specific magneto-extraction-aided LAMP for fluorescence and electrochemical SARS-CoV-2 nucleic acid detectionArticle Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 변종, 치료기술, 치료제, [키워드] amplification approach circumvent clinically Disadvantages Electrochemical LAMP Host in silico inhibitor investigated isothermal isothermal amplification Magnetic sequence-specific target enrichment molecular NAAT nucleic acid Nucleic acid detection PCR polymerase probe qRT-PCR Quantitative Quantitative real-time LAMP real-time reverse transcriptase restrict SARS-CoV-2 SARS-CoV-2 nucleic acid SARS-CoV-2 variant SARS-CoV-2. sensitivity sequence serum turn [DOI] 10.1016/j.talanta.2022.123809 PMC 바로가기
Selection of Ideal Reference Genes for Gene Expression Analysis in COVID-19 and MucormycosisArticle Published on 2022-12-212023-07-10 Journal: Microbiology Spectrum [Category] COVID19(2023년), [키워드] COVID-19 CypA Gene Expression IL-6 Mucormycosis Nrf2 qRT-PCR reference gene. SARS-CoV-2 [DOI] 10.1128/spectrum.01656-22 PMC 바로가기
Viral load dynamics of SARS-CoV-2 Delta and Omicron variants following multiple vaccine doses and previous infectionArticle Published on 2022-11-072022-11-16 Journal: Nature Communications [Category] SARS, 변종, [키워드] affected affecting booster can be used COVID-19 cycle threshold Delta Delta wave demonstrated effective Effectiveness implication individuals Infection Laboratory less morbidity and mortality natural infection omicron pathogen public health qRT-PCR reduce SARS-CoV-2 significantly sustained the vaccine Transmissibility vaccination Vaccine vaccine dose variant Viral load while [DOI] 10.1038/s41467-022-33096-0 PMC 바로가기
Long-Term Wastewater Surveillance for SARS-CoV-2: One-Year Study in BrazilArticle Published on 2022-10-252023-07-10 Journal: Viruses [Category] COVID19(2023년), [키워드] Epidemiology qRT-PCR SARS-CoV-2 Wastewater. [DOI] 10.3390/v14112333 PMC 바로가기
Construction and evaluation of a self-replicative RNA vaccine against SARS-CoV-2 using yellow fever virus repliconArticle Published on 2022-10-202022-11-15 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 진단, [키워드] acute respiratory syndrome antibody Antigen C57BL/6 caused cells co-expressed construction coronavirus coronavirus disease COVID-19 effective vaccine Efficacy Electroporation elicit ENCODE ENhance evaluated Fever gene encoding Host host genome immune response Immunity IMPROVE Infection Innate immunity kidney mice mRNA vaccine pandemic Protein qRT-PCR Replication replicon responses RNA RNA vaccine robust S protein expression Safe SARS-CoV-2 significantly higher single dose Specific T-cell response T-cell Response the S protein Vaccine vaccine administration vaccine platform virus [DOI] 10.1371/journal.pone.0274829 PMC 바로가기
Point-of-care detection of SARS-CoV-2 antigen among symptomatic vs. asymptomatic persons: Testing for COVID-19 vs. infectivityArticle Published on 2022-10-172022-11-15 Journal: Frontiers in Public Health [Category] COVID19(2023년), SARS, 치료기술, [키워드] 95% confidence interval acute respiratory syndrome Ag-RDT Antigen Asymptomatic Buenos Aires Care caused Concordance Contact coronavirus disease Coronavirus-2 COVID-19 COVID-19 patient detect diagnose diagnostic evaluate false-negative hypothetical identify individual Infectious virus Isolation pandemic Participants PCR point-of-care positive public health qRT-PCR rapid antigen testing real-time RT-PCR SARS-CoV-2 SARS-CoV-2 antigen SARS-COV-2 infection SARS-CoV-2 RNA SARS-CoV-2 testing subjects Surveillance symptomatic t -value Testing threshold Transmission utility viral kinetics viral kinetics. was obtained [DOI] 10.3389/fpubh.2022.995249 PMC 바로가기
PrimedSherlock: a tool for rapid design of highly specific CRISPR-Cas12 crRNAsArticle Published on 2022-10-142022-11-15 Journal: BMC Bioinformatics [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] assays automated benefit bioinformatics Cas12 nuclease assay Cas12a Combination conserved CRISPR CRISPR-Ca CRISPR-Cas12 Critical demonstrated Dengue diagnostic diagnostics Efficiency facilitated FDA approval field Field forward diagnostics flavivirus genomes genomic HIV In-vitro Intensive isothermal isothermal amplification manual curation Nile nucleases nucleic acid offer parameter Pathogens platform polymorphism PrimedRPA PrimedSherlock primer primer pair Primers qRT-PCR regions RNA robust SARS-CoV-2 Serological assay serotype SHERLOCK significantly specificity strain Strains target targets Vector-borne disease West West Nile wildtype Zika Zika. [DOI] 10.1186/s12859-022-04968-5 PMC 바로가기
Comparison and Harmonization of Different Semi-Automated and Automated qRT-PCR Assays in the Assessment of SARS-CoV-2Article Published on 2022-10-122022-11-15 Journal: Viruses [Category] COVID19(2023년), SARS, 치료기술, [키워드] Analysis Applying assays assessment automated carried comparability Concentration COVID-19 cycle threshold Cycle threshold value detectable diagnostics FIVE harmonization highlight identify infected individuals Isolation Laboratory lowest mandatory oropharyngeal swab Patient qRT-PCR qRT-PCR assay Qualitative Quantitative quantitative reverse transcriptase-polymerase chain reaction RIDA SARS-CoV-2 SARS-CoV-2 qRT-PCR sensitivity significant difference significantly the median Viral load Viral load. [DOI] 10.3390/v14102239 PMC 바로가기
A fast, ultrasensitive SERS immunoassay to detect SARS-CoV-2 in salivaArticle Published on 2022-10-092022-11-15 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 변종, 진단, 치료기술, [키워드] Alpha antibody Antigen Concentration COVID-19 COVID-19 pandemic detect Diagnosis diagnostics disease transmission E. coli expressed Genome immunoassay inactivated less MERS Omicron variants point-of-care qRT-PCR Raman spectroscopy recombinant Saliva SARS-CoV-2 SARS-CoV-2 spike proteins SARS-CoV-2 virus scattering scFv Single chain variable fragment (scFv) Spike protein Spike proteins Surface-enhanced Raman scattering (SERS). tested the SARS-CoV-2 the SARS-CoV-2 virus [DOI] 10.1016/j.aca.2022.340290 PMC 바로가기
A paper-based optical sensor for the screening of viruses through the cysteine residues of their surface proteins: A proof of concept on the detection of coronavirus infection표면 단백질의 시스테인 잔기를 통한 바이러스 스크리닝을 위한 종이 기반 광학 센서: 코로나바이러스 감염 감지에 대한 개념 증명Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus BENZOTHIAZOLE Biothiol cause Chain Reaction clinical study Colorimetric assay complex Concentration coronavirus Coronavirus infection current cysteine cysteine residue Disulfide disulfide bond Filter Paper group Health Human immunodeficiency virus human Papilloma virus immunodeficiency intensity lab lack limit of detection linear relationship logarithm lowest naked eye nasopharyngeal swabs optical pandemic paper Paper based sensor papilloma pathology Patient pink color polymerase chain polymerase chain reaction proof proof of concept Protein Proteins qRT-PCR Rapid reduced required reverse transcription reverse transcription polymerase chain reaction S1 protein Saliva SARS-CoV-2 SARS-CoV-2. screening method severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 specimens Spike protein subunit sulfhydryl the S1 protein time-consuming virus virus viruses [DOI] 10.1016/j.talanta.2022.123630 PMC 바로가기 [Article Type] Article